​Determination of Serogroup and Lytic Activities of Bacteriophages Isolated from Phage Plaques in Staphylococcus aureus Cultures Identified from Sheep Milk with Mastitis

Backgorund: Bacteriophages are closely related to the evolution and virulence of some important bacterial pathogens. Due their highly significant roles in pathogenesis virulence, S. aureus bacteriophages frequently studied. grouped into two main categories depending on life cycles. There consistently lytic phages (virulent) temperate phages. This study aimed isolate determine phage serogroups from plaques cultures order show if they or lysogenic, latter plays a major role horizontal gene transfer. Methods: A total 234 isolates were recovered milk samples cases with gangrenous mastitis sheep. Staphylococcal determined based type serogroup by PCR using specific primers. Result: Our allowed us isolated bacteriophages. Two stock included only one while others more than serotypes needed further purification Fa, L D not study. Present work revealed that all phages, which play